Molecular.asp

Molecular biology laboratory protocols: plant genotyping

Cover
Credits
Foreword
Abbreviations

Table of Contents

1. Plant DNA Extraction
Small-Scale
Medium-Scale

2. Mini-Scale Plasmid Extraction

3. PCR Amplification of Plasmid Inserts

4. Non-Radioactive RFLP Analysis

5. Random Amplified Polymorphic DNA (RAPD)

6. Single Sequence Repeats (SSR) or Microsatellites

7. Amplified Fragment Length Polymorphism (AFLP)

8. Non-Radioactive Amplified Fragment Length Polymorphism

9. DNA Gel Electrophoresis
Preparation of Recycled Agarose Gels (1.4%)
Polyacrylamide Gel for DNA Electrophoresis
Silver-Staining the Sequencing Gel

10. Purification of TAQ Polymerase (Short Protocol)