Materials
Molecular biology laboratory protocols: plant genotyping
Cover
Credits
Foreword
Abbreviations
Table of Contents
1.
Plant DNA Extraction
Small-Scale
Medium-Scale
2.
Mini-Scale Plasmid Extraction
3.
PCR Amplification of Plasmid Inserts
4.
Non-Radioactive RFLP Analysis
5.
Random Amplified Polymorphic DNA (RAPD)
6.
Single Sequence Repeats (SSR) or Microsatellites
7.
Amplified Fragment Length Polymorphism (AFLP)
8.
Non-Radioactive Amplified Fragment Length Polymorphism
9.
DNA Gel Electrophoresis
Preparation of Recycled Agarose Gels (1.4%)
Polyacrylamide Gel for DNA Electrophoresis
Silver-Staining the Sequencing Gel
10.
Purification of TAQ Polymerase (Short Protocol)
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