Training / Materials
NCM-ELISA
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Training materials
for NCM-ELISA: Instructor's manual and video. 1998. CIP. $20 (Available in Chinese, English, and Spanish) |
This new set of new training
materials includes a video and instruction
manual on the use of CIP's new kit for
NCM-ELISA (enzyme-linked immunosorbent
assay on nitrocellulose membrane using
enriched samples). The NCM-ELISA Kit helps
detect Ralstonia solanacearum, the agent
that causes bacterial wilt or "brown
rot" in potato. To detect R. solanacearum
in latently infected tubers (those without
any visible symptoms), tuber extracts
must be incubated in a semi-selective
broth (modified SMSA; Elphinstone et al
1996, EPPO/OEPP Bulletin, 26:663-678)
prior to testing. This enrichment procedure
increases the sensitivity of the assay
by nearly one million. As few as 10 bacteria
per ml of extract can be detected with
this method (versus 106-107 bact./ml without
enrichment).
Post-enrichment NCM-ELISA is as sensitive as the Double-Antibody Sandwich ELISA (DAS-ELISA), but is much easier and quicker. Since samples and reagents are supported by nitrocellulose membrane instead of microtitre plates, they can be stored for several weeks before testing--or sent to outside labs. After NCM-ELISA, the membrane is stable and can be stored for extended periods. The NCM-ELISA Kit can be used to monitor R. solanacearum in potato tubers-- essential for seed production systems and varietal evaluation of resistance to bacterial wilt. It can also be used with the roots and stems of potato and other plants for research on disease epidemiology (eg, the survival and spread of R. solanacearum). How to order For more information on the use of this kit, please contact Dr. Sylvie Priou in CIP's Crop Protection Department. |
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