This training manual describes the following
protocols for plant genotyping, all used successfully for potatoes,
sweetpotatoes, and Andean roots and tubers at CIP's molecular
biology laboratory:
- Medium- and small-scale plant DNA extraction;
- Mini-scale plasmid extraction;
- PCR amplification of plasmid inserts;
- Non-radioactive RFLP analysis;
- Random amplified polymorphic DNA (RAPD);
- Single sequence repeats (SSR) or
microsatellites;
- Amplified fragment length polymorphism (AFLP);
- Non-radioactive amplified fragment length
polymorphism;
- DNA gel electrophoresis; and
- Purification of TAQ polymerase (short
protocol).
For more information about content or use of
these protocols, contact CIP's molecular
biology laboratory.
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