This new set of new training materials includes a video and instruction manual on the use of CIP's new kit for NCM-ELISA (enzyme-linked immunosorbent assay on nitrocellulose membrane using enriched samples). The NCM-ELISA Kit helps detect Ralstonia solanacearum, the agent that causes bacterial wilt or "brown rot" in potato. To detect R. solanacearum in latently infected tubers (those without any visible symptoms), tuber extracts must be incubated in a semi-selective broth (modified SMSA; Elphinstone et al 1996, EPPO/OEPP Bulletin, 26:663-678) prior to testing. This enrichment procedure increases the sensitivity of the assay by nearly one million. As few as 10 bacteria per ml of extract can be detected with this method (versus 106-107 bact./ml without enrichment).

Post-enrichment NCM-ELISA is as sensitive as the Double-Antibody Sandwich ELISA (DAS-ELISA), but is much easier and quicker. Since samples and reagents are supported by nitrocellulose membrane instead of microtitre plates, they can be stored for several weeks before testing--or sent to outside labs. After NCM-ELISA, the membrane is stable and can be stored for extended periods.

The NCM-ELISA Kit can be used to monitor R. solanacearum in potato tubers-- essential for seed production systems and varietal evaluation of resistance to bacterial wilt. It can also be used with the roots and stems of potato and other plants for research on disease epidemiology (eg, the survival and spread of R. solanacearum).

How to order

For more information on the use of this kit, please contact Dr. Sylvie Priou in CIP's Crop Protection Department.